MetaCyc Pathway: gluconeogenesis I in Escherichia coli ECRC98

Add experiment(s):


Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD+→pyruvate + CO2 + NADH (EC 1.1.1.38)
JDDGAC_03850 (maeA)
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
JDDGAC_03850 (maeA)
JDDGAC_09905 (hcxB)
JDDGAC_21420 (mdh)
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→pyruvate + CO2 + NADPH (EC 1.1.1.40)
JDDGAC_25950 (maeB)
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→phosphoenolpyruvate + ADP + CO2 (EC 4.1.1.49)
JDDGAC_20675 (pckA)
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
JDDGAC_01720 (ppsA)
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11)
JDDGAC_23940 (eno)
2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12)
JDDGAC_10390 (gpmA)
JDDGAC_14750
JDDGAC_19360 (gpmM)
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
JDDGAC_23020 (pgk)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADH + H+→D-glyceraldehyde 3-phosphate + NAD+ + phosphate (EC 1.2.1.12)
JDDGAC_01305 (gapA)
JDDGAC_04185 (gap)
Fructose-bisphosphate aldolase (in reverse):
D-glyceraldehyde 3-phosphate + glycerone phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
JDDGAC_16365 (fba)
JDDGAC_20120 (fba)
JDDGAC_23025 (fbaA)
JDDGAC_28215 (fbaB)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
JDDGAC_10335 (ybhA)
JDDGAC_12280 (phoA)
JDDGAC_15585 (fbp)
JDDGAC_17405 (glpX)
JDDGAC_22975 (glpX)
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
JDDGAC_17435 (tpiA)
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
JDDGAC_16800 (pgi)
JDDGAC_23585 (kduI)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information