MetaCyc Pathway: mixed acid fermentation in Escherichia coli ECRC98

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
JDDGAC_17220 (ppc)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
JDDGAC_00895 (pyk)
JDDGAC_01865 (pykF)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
JDDGAC_04285 (ldhA)
JDDGAC_27605 (dld)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
JDDGAC_03850 (maeA)
JDDGAC_09905 (hcxB)
JDDGAC_21420 (mdh)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
JDDGAC_09120 (pflB)
JDDGAC_09325 (pflA)
JDDGAC_09770 (ybiY)
JDDGAC_09775
JDDGAC_09780 (pflD)
JDDGAC_17240 (pflC)
JDDGAC_17245 (pflD)
JDDGAC_22050 (tdcE)
JDDGAC_25010 (grcA)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
JDDGAC_05970 (adhE)
JDDGAC_12420 (mhpF)
JDDGAC_25990 (eutE)
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
JDDGAC_10615 (gltA)
formate + H+→CO2 + H2
JDDGAC_16405 (fdhF)
JDDGAC_24240 (hycB)
JDDGAC_24245 (hycC)
JDDGAC_24250 (hycD)
JDDGAC_25835 (hyfC)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
JDDGAC_01870 (fumD)
JDDGAC_02235 (fumB)
JDDGAC_02240 (fumC)
JDDGAC_10550 (ttdA)
JDDGAC_16160 (fumB)
JDDGAC_22980 (fumE)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
JDDGAC_25975 (pta)
JDDGAC_26755 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
JDDGAC_00925 (purT)
JDDGAC_22045 (tdcD)
JDDGAC_25960 (eutP)
JDDGAC_25965 (eutQ)
JDDGAC_26760 (ackA)
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
JDDGAC_05120 (acnA)
JDDGAC_10310 (ybhJ)
JDDGAC_14075 (acnB)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
JDDGAC_03855 (adhP)
JDDGAC_05970 (adhE)
JDDGAC_12395 (frmA)
JDDGAC_12580 (yahK)
JDDGAC_15410 (ahr)
JDDGAC_19485 (yiaY)
JDDGAC_21340 (acuI)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
JDDGAC_15980 (frdA)
JDDGAC_15985 (frdB)
JDDGAC_15990
JDDGAC_15995 (frdD)
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
JDDGAC_05120 (acnA)
JDDGAC_10310 (ybhJ)
JDDGAC_14075 (acnB)
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
JDDGAC_06605 (icd)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information