MetaCyc Pathway: superpathway of Clostridium acetobutylicum solventogenic fermentation in Escherichia coli ECRC62

Add experiment(s):


Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BNILDI_18000 (nifJ)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
BNILDI_12535 (eutE)
BNILDI_18590 (adhE)
BNILDI_23090 (mhpF)
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BNILDI_05150 (fadA)
BNILDI_10280 (yqeF)
BNILDI_13105 (fadI)
BNILDI_17485
BNILDI_17900 (paaJ)
Butyrate--acetoacetate CoA-transferase (in reverse):
acetoacetyl-CoA + butanoate→acetoacetate + butanoyl-CoA (EC 2.8.3.9)
No genes
Butyrate--acetoacetate CoA-transferase (in reverse):
acetoacetyl-CoA + acetate→acetoacetate + acetyl-CoA (EC 2.8.3.9)
BNILDI_16500 (ydiF)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
BNILDI_02020 (acuI)
BNILDI_03780 (yiaY)
BNILDI_07480 (ahr)
BNILDI_17525 (adhP)
BNILDI_18590 (adhE)
BNILDI_23065 (frmA)
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
BNILDI_05155 (fadB)
BNILDI_13110 (fadJ)
BNILDI_17490
Acetoacetate decarboxylase:
acetoacetate + H+→acetone + CO2 (EC 4.1.1.4)
No genes
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
BNILDI_05155 (fadB)
BNILDI_13110 (fadJ)
BNILDI_17490
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information