MetaCyc Pathway: heterolactic fermentation in Escherichia coli ECRC62

Add experiment(s):


Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
BNILDI_05375 (yihV)
BNILDI_13005 (cscK)
BNILDI_22910 (mak)
Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
BNILDI_12860 (glk)
BNILDI_22910 (mak)
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
BNILDI_06185 (pgi)
BNILDI_10285 (kduI)
Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP+→6-phospho D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.49)
BNILDI_15640 (zwf)
6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H2O→D-gluconate 6-phosphate + H+ (EC 3.1.1.31)
BNILDI_21100 (pgl)
D-gluconate 6-phosphate + NAD(P)+→CO2 + D-ribulose 5-phosphate + NAD(P)H (EC 1.1.1.351)
No genes
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
BNILDI_02600 (dam)
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
BNILDI_16045 (gapA)
BNILDI_17830 (gap)
Phosphate acetyltransferase (in reverse):
acetyl phosphate + coenzyme A→acetyl-CoA + phosphate (EC 2.3.1.8)
BNILDI_12520 (pta)
BNILDI_13330 (pta)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
BNILDI_12535 (eutE)
BNILDI_18590 (adhE)
BNILDI_23090 (mhpF)
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
BNILDI_09840 (pgk)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
BNILDI_02020 (acuI)
BNILDI_03780 (yiaY)
BNILDI_07480 (ahr)
BNILDI_17525 (adhP)
BNILDI_18590 (adhE)
BNILDI_23065 (frmA)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
BNILDI_21160 (gpmA)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
BNILDI_10655 (eno)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BNILDI_15630 (pyk)
BNILDI_16600 (pykF)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
BNILDI_14140 (dld)
BNILDI_17985 (ldhA)
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
BNILDI_00040 (ykgF)
BNILDI_00045 (ykgE)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information