MetaCyc Pathway: mixed acid fermentation in Escherichia coli ECRC62

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
BNILDI_05785 (ppc)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BNILDI_15630 (pyk)
BNILDI_16600 (pykF)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
BNILDI_14140 (dld)
BNILDI_17985 (ldhA)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BNILDI_01940 (mdh)
BNILDI_17520 (maeA)
BNILDI_20900 (hcxB)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
BNILDI_01330 (tdcE)
BNILDI_05765 (pflC)
BNILDI_11820 (grcA)
BNILDI_20325 (pflB)
BNILDI_20330 (pflA)
BNILDI_20765 (ybiY)
BNILDI_20770 (ybiW)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
BNILDI_12535 (eutE)
BNILDI_18590 (adhE)
BNILDI_23090 (mhpF)
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
BNILDI_21340 (gltA)
formate + H+→CO2 + H2
BNILDI_06500 (fdhF)
BNILDI_10970 (hycB)
BNILDI_10975 (hycC)
BNILDI_10980 (hycD)
BNILDI_12360 (hyfC)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
BNILDI_06700 (fumB)
BNILDI_09800 (fumE)
BNILDI_16605 (fumD)
BNILDI_16940 (fumB)
BNILDI_16945 (fumC)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BNILDI_12520 (pta)
BNILDI_13330 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BNILDI_01335 (tdcD)
BNILDI_12505 (eutP)
BNILDI_12510 (eutQ)
BNILDI_13335 (ackA)
BNILDI_15655 (purT)
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
BNILDI_08785 (acnB)
BNILDI_18390 (acnA)
BNILDI_21080 (acnA)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
BNILDI_02020 (acuI)
BNILDI_03780 (yiaY)
BNILDI_07480 (ahr)
BNILDI_17525 (adhP)
BNILDI_18590 (adhE)
BNILDI_23065 (frmA)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
BNILDI_06870 (frdD)
BNILDI_06875
BNILDI_06880 (frdB)
BNILDI_06885 (frdA)
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
BNILDI_08785 (acnB)
BNILDI_18390 (acnA)
BNILDI_21080 (acnA)
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
BNILDI_19050 (icd)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information