MetaCyc Pathway: mixed acid fermentation in Escherichia coli ECRC102

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
NIAGMN_11345 (ppc)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
NIAGMN_00775 (pykF)
NIAGMN_01740 (pyk)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
NIAGMN_21415 (dld)
NIAGMN_25930 (ldhA)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
NIAGMN_04315 (hcxB)
NIAGMN_15555 (mdh)
NIAGMN_25490 (maeA)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
NIAGMN_03230 (pflB)
NIAGMN_03235 (pflA)
NIAGMN_04180 (ybiY)
NIAGMN_04185
NIAGMN_04190 (pflD)
NIAGMN_11365 (pflC)
NIAGMN_11370 (pflD)
NIAGMN_16180 (tdcE)
NIAGMN_19115 (grcA)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
NIAGMN_06820 (mhpF)
NIAGMN_19815 (eutE)
NIAGMN_27630 (adhE)
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
NIAGMN_05010 (gltA)
formate + H+→CO2 + H2
NIAGMN_10615 (fdhF)
NIAGMN_18345 (hycB)
NIAGMN_18350 (hycC)
NIAGMN_18355 (hycD)
NIAGMN_19660 (hyfC)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
NIAGMN_00425 (fumC)
NIAGMN_00430 (fumB)
NIAGMN_00770 (fumD)
NIAGMN_04945 (ttdA)
NIAGMN_10370 (fumB)
NIAGMN_17115 (fumE)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
NIAGMN_19800 (pta)
NIAGMN_20560 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
NIAGMN_01710 (purT)
NIAGMN_16175 (tdcD)
NIAGMN_19785 (eutP)
NIAGMN_19790 (eutQ)
NIAGMN_20565 (ackA)
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
NIAGMN_04705 (ybhJ)
NIAGMN_08330 (acnB)
NIAGMN_26745 (acnA)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
NIAGMN_06795 (frmA)
NIAGMN_06980 (yahK)
NIAGMN_09620 (ahr)
NIAGMN_13615 (yiaY)
NIAGMN_15475 (acuI)
NIAGMN_25495 (adhP)
NIAGMN_27630 (adhE)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
NIAGMN_10190 (frdA)
NIAGMN_10195 (frdB)
NIAGMN_10200
NIAGMN_10205 (frdD)
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
NIAGMN_04705 (ybhJ)
NIAGMN_08330 (acnB)
NIAGMN_26745 (acnA)
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
NIAGMN_24905 (icd)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information