MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Escherichia coli ECRC101

Add experiment(s):


L-lactate dehydrogenase:
(S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
MCAODC_28325 (ykgF)
MCAODC_28330 (ykgE)
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
MCAODC_04115 (pflC)
MCAODC_04120 (pflD)
MCAODC_08930 (tdcE)
MCAODC_11875 (grcA)
MCAODC_24900 (pflB)
MCAODC_24905 (pflA)
MCAODC_25350 (ybiY)
MCAODC_25355
MCAODC_25360 (pflD)
formate + H+→H2 + CO2
MCAODC_03280 (fdhF)
MCAODC_11100 (hycB)
MCAODC_11105 (hycC)
MCAODC_11110 (hycD)
MCAODC_12420 (hyfC)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
MCAODC_08315 (mdh)
MCAODC_16580 (maeA)
MCAODC_25485 (hcxB)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
MCAODC_12560 (pta)
MCAODC_13830 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
MCAODC_08925 (tdcD)
MCAODC_12545 (eutP)
MCAODC_12550 (eutQ)
MCAODC_13835 (ackA)
MCAODC_19055 (purT)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
MCAODC_03035 (fumB)
MCAODC_09870 (fumE)
MCAODC_17760 (fumC)
MCAODC_17765 (fumB)
MCAODC_18115 (fumD)
MCAODC_26115 (ttdA)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
MCAODC_02855 (frdA)
MCAODC_02860 (frdB)
MCAODC_02865
MCAODC_02870 (frdD)
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
MCAODC_09950 (scpC)
Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
MCAODC_09965 (scpA)
Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information