MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Escherichia coli ECRC101

Add experiment(s):


Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
MCAODC_06320 (mtlD)
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
MCAODC_03700 (fabG)
MCAODC_11195 (srlD)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
MCAODC_02450 (fbp)
MCAODC_04280 (glpX)
MCAODC_09865 (glpX)
MCAODC_25900 (ybhA)
MCAODC_27850 (phoA)
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
MCAODC_04330 (pfkA)
MCAODC_18375 (pfkB)
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
MCAODC_03240 (fba)
MCAODC_07010 (fba)
MCAODC_09915 (fbaA)
MCAODC_21055 (fbaB)
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
MCAODC_04310 (tpiA)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
MCAODC_16240 (gap)
MCAODC_18680 (gapA)
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
MCAODC_09910 (pgk)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
MCAODC_01670
MCAODC_06245 (gpmM)
MCAODC_25955 (gpmA)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
MCAODC_25955 (gpmA)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
MCAODC_10800 (eno)
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
MCAODC_18265 (ppsA)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
MCAODC_18120 (pykF)
MCAODC_19085 (pyk)
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
MCAODC_28325 (ykgF)
MCAODC_28330 (ykgE)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
MCAODC_04115 (pflC)
MCAODC_04120 (pflD)
MCAODC_08930 (tdcE)
MCAODC_11875 (grcA)
MCAODC_24900 (pflB)
MCAODC_24905 (pflA)
MCAODC_25350 (ybiY)
MCAODC_25355
MCAODC_25360 (pflD)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
MCAODC_12575 (eutE)
MCAODC_21655 (adhE)
MCAODC_27995 (mhpF)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
MCAODC_12560 (pta)
MCAODC_13830 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
MCAODC_08925 (tdcD)
MCAODC_12545 (eutP)
MCAODC_12550 (eutQ)
MCAODC_13835 (ackA)
MCAODC_19055 (purT)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
MCAODC_02275 (ahr)
MCAODC_06370 (yiaY)
MCAODC_08235 (acuI)
MCAODC_16575 (adhP)
MCAODC_21655 (adhE)
MCAODC_27970 (frmA)
MCAODC_28155 (yahK)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information