MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Escherichia coli ECRC101

Add experiment(s):


L-lactate dehydrogenase:
(S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
OKFHMN_09100 (ykgF)
OKFHMN_09105 (ykgE)
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
OKFHMN_05510 (pflB)
OKFHMN_05515 (pflA)
OKFHMN_06135 (ybiY)
OKFHMN_06140
OKFHMN_06145 (pflD)
OKFHMN_13615 (pflC)
OKFHMN_13620 (pflD)
OKFHMN_18415 (tdcE)
OKFHMN_21345 (grcA)
formate + H+→H2 + CO2
OKFHMN_12770 (fdhF)
OKFHMN_20575 (hycB)
OKFHMN_20580 (hycC)
OKFHMN_20585 (hycD)
OKFHMN_21890 (hyfC)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
OKFHMN_01360 (maeA)
OKFHMN_06270 (hcxB)
OKFHMN_17795 (mdh)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
OKFHMN_22030 (pta)
OKFHMN_22790 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
OKFHMN_18410 (tdcD)
OKFHMN_22015 (eutP)
OKFHMN_22020 (eutQ)
OKFHMN_22795 (ackA)
OKFHMN_26350 (purT)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
OKFHMN_06895 (ttdA)
OKFHMN_12525 (fumB)
OKFHMN_19350 (fumE)
OKFHMN_27295 (fumD)
OKFHMN_27645 (fumB)
OKFHMN_27650 (fumC)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
OKFHMN_12345 (frdA)
OKFHMN_12350 (frdB)
OKFHMN_12355
OKFHMN_12360 (frdD)
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
OKFHMN_19430 (scpC)
Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
OKFHMN_19445 (scpA)
Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information