MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Escherichia coli ECRC101

Add experiment(s):


2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
OKFHMN_13785 (fpr)
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate (EC 1.2.1.13)
No genes
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
OKFHMN_13810 (tpiA)
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
OKFHMN_12730 (fba)
OKFHMN_16490 (fba)
OKFHMN_19395 (fbaA)
OKFHMN_24105 (fbaB)
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)
OKFHMN_12730 (fba)
OKFHMN_16490 (fba)
OKFHMN_19395 (fbaA)
OKFHMN_24105 (fbaB)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
OKFHMN_06680 (ybhA)
OKFHMN_08625 (phoA)
OKFHMN_11945 (fbp)
OKFHMN_13780 (glpX)
OKFHMN_19345 (glpX)
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
OKFHMN_19325 (tkt)
OKFHMN_21995 (tkt)
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
OKFHMN_19325 (tkt)
OKFHMN_21995 (tkt)
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
OKFHMN_19455 (rpiA)
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
OKFHMN_17130 (dam)
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
OKFHMN_17250 (prkB)
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
No genes
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
OKFHMN_06735 (gpmA)
OKFHMN_11155
OKFHMN_15735 (gpmM)
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
OKFHMN_19390 (pgk)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate (EC 4.2.1.11)
OKFHMN_20275 (eno)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
OKFHMN_26320 (pyk)
OKFHMN_27290 (pykF)
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
OKFHMN_10490 (lpdA)
OKFHMN_10495 (aceF)
OKFHMN_10500 (aceE)
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
OKFHMN_14250 (fadA)
OKFHMN_19915 (yqeF)
OKFHMN_22555 (fadI)
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
OKFHMN_14245 (fadB)
OKFHMN_22560 (fadJ)
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
OKFHMN_14245 (fadB)
OKFHMN_22560 (fadJ)
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
OKFHMN_09780 (fadE)
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information