MetaCyc Pathway: γ-glutamyl cycle in Escherichia coli ECRC101

5-oxoprolinase (ATP-hydrolyzing): 5-oxo-L-proline + ATP + 2 H2O→L-glutamate + ADP + H+ + phosphate (EC 3.5.2.9)

OKFHMN_06995 (pxpA)

OKFHMN_07000 (pxpC)

OKFHMN_07005 (pxpB)

Glutamate--cysteine ligase: L-glutamate + L-cysteine + ATP→γ-L-glutamyl-L-cysteine + ADP + H+ + phosphate (EC 6.3.2.2)

OKFHMN_07655 (ybdK)

OKFHMN_20760 (gshA)

Glutathione synthase: γ-L-glutamyl-L-cysteine + ATP + glycine→glutathione + ADP + H+ + phosphate (EC 6.3.2.3)

OKFHMN_19275 (gshB)

Gamma-glutamyltransferase: glutathione + a proteinogenic amino acid→an α-(γ-L-glutamyl)-L-amino acid + L-cysteinylglycine (EC 2.3.2.2)

OKFHMN_16800 (ggt)

an α-(γ-L-glutamyl)-L-amino acid→5-oxo-L-proline + an L-amino acid (EC 4.3.2.9)

No genes

Cytosol nonspecific dipeptidase: L-cysteinylglycine + H2O→L-cysteine + glycine (EC 3.4.13.18)

OKFHMN_05365 (pepN)

OKFHMN_09700 (pepD)

OKFHMN_11790 (pepA)

OKFHMN_21655 (pepB)

OKFHMN_22340 (ypdF)

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information