MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Escherichia coli ECRC101

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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
OKFHMN_01580 (ydcR)
OKFHMN_05385 (aspC)
OKFHMN_11450 (yjiR)
OKFHMN_20855 (aRO8)
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
OKFHMN_11110 (thrA)
OKFHMN_13180 (lysC)
OKFHMN_13670 (metL)
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
OKFHMN_16875 (asd)
OKFHMN_22665 (usg)
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
OKFHMN_11110 (thrA)
OKFHMN_13670 (metL)
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
OKFHMN_11105 (thrB)
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
OKFHMN_11100 (thrC)
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
OKFHMN_14635 (ilvA)
OKFHMN_18400 (tdcB)
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
OKFHMN_14635 (ilvA)
OKFHMN_18400 (tdcB)
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
OKFHMN_11875 (ridA)
OKFHMN_14635 (ilvA)
OKFHMN_18400 (tdcB)
OKFHMN_18420 (tdcF)
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
OKFHMN_10695 (ilvN)
OKFHMN_10700 (ilvI)
OKFHMN_14650
OKFHMN_14655 (ilvG)
OKFHMN_15150
OKFHMN_15155 (ilvN)
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
OKFHMN_14625 (ilvC)
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
OKFHMN_14640 (ilvD)
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
OKFHMN_14645 (ilvE)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information