MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Escherichia coli ECRC101

Add experiment(s):


Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
OKFHMN_15810 (mtlD)
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
OKFHMN_13200 (fabG)
OKFHMN_20670 (srlD)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
OKFHMN_06680 (ybhA)
OKFHMN_08625 (phoA)
OKFHMN_11945 (fbp)
OKFHMN_13780 (glpX)
OKFHMN_19345 (glpX)
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
OKFHMN_13830 (pfkA)
OKFHMN_27035 (pfkB)
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
OKFHMN_12730 (fba)
OKFHMN_16490 (fba)
OKFHMN_19395 (fbaA)
OKFHMN_24105 (fbaB)
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
OKFHMN_13810 (tpiA)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
OKFHMN_01695 (gap)
OKFHMN_26730 (gapA)
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
OKFHMN_19390 (pgk)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
OKFHMN_06735 (gpmA)
OKFHMN_11155
OKFHMN_15735 (gpmM)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
OKFHMN_06735 (gpmA)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
OKFHMN_20275 (eno)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
OKFHMN_26320 (pyk)
OKFHMN_27290 (pykF)
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
OKFHMN_09100 (ykgF)
OKFHMN_09105 (ykgE)
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
OKFHMN_27145 (ppsA)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
OKFHMN_05510 (pflB)
OKFHMN_05515 (pflA)
OKFHMN_06135 (ybiY)
OKFHMN_06140
OKFHMN_06145 (pflD)
OKFHMN_13615 (pflC)
OKFHMN_13620 (pflD)
OKFHMN_18415 (tdcE)
OKFHMN_21345 (grcA)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
OKFHMN_08765 (mhpF)
OKFHMN_22045 (eutE)
OKFHMN_28605 (adhE)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
OKFHMN_22030 (pta)
OKFHMN_22790 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
OKFHMN_18410 (tdcD)
OKFHMN_22015 (eutP)
OKFHMN_22020 (eutQ)
OKFHMN_22795 (ackA)
OKFHMN_26350 (purT)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
OKFHMN_01365 (adhP)
OKFHMN_08740 (frmA)
OKFHMN_08925 (yahK)
OKFHMN_11770 (ahr)
OKFHMN_15855 (yiaY)
OKFHMN_17715 (acuI)
OKFHMN_28605 (adhE)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information