MetaCyc Pathway: superpathway of L-threonine metabolism in Escherichia coli ECOR38

Add experiment(s):


Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
HEPCGN_13345 (ilvA)
HEPCGN_17235 (tdcB)
L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177)
No genes
L-threonine 3-dehydrogenase:
L-threonine + NAD+→L-2-amino-3-oxobutanoate + NADH + H+ (EC 1.1.1.103)
HEPCGN_14555 (tdh)
L-threonine→acetaldehyde + glycine (EC 4.1.2.48; 4.1.2.5)
HEPCGN_02165 (ltaE)
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2 (EC 4.1.1.81)
No genes
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10)
HEPCGN_07390 (mhpF)
HEPCGN_14195 (adhE)
HEPCGN_21215 (eutE)
HEPCGN_25070 (adhE)
Glycine C-acetyltransferase (in reverse):
L-2-amino-3-oxobutanoate + coenzyme A→glycine + acetyl-CoA (EC 2.3.1.29)
HEPCGN_14550 (kbl)
HEPCGN_17965
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HEPCGN_13345 (ilvA)
HEPCGN_17235 (tdcB)
L-threonine 3-dehydrogenase:
L-2-amino-3-oxobutanoate + H+→aminoacetone + CO2 (spontaneous) (EC 1.1.1.103; 1.1.1.381)
HEPCGN_14555 (tdh)
Primary-amine oxidase:
aminoacetone + dioxygen + H2O→methylglyoxal + ammonium + hydrogen peroxide (EC 1.4.3.21)
No genes
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HEPCGN_10070 (ridA)
HEPCGN_13345 (ilvA)
HEPCGN_17235 (tdcB)
HEPCGN_17255 (tdcF)
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
HEPCGN_08675 (ilvN)
HEPCGN_08680 (ilvI)
HEPCGN_13360
HEPCGN_13365 (ilvG)
HEPCGN_13885
HEPCGN_13890 (ilvN)
Formate C-acetyltransferase (in reverse):
2-oxobutanoate + coenzyme A→propanoyl-CoA + formate (EC 2.3.1.54)
HEPCGN_01895 (ybiW)
HEPCGN_12265 (pflD)
HEPCGN_14180 (cutC)
HEPCGN_17250 (tdcE)
HEPCGN_20470 (grcA)
HEPCGN_23010 (pflB)
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
HEPCGN_13335 (ilvC)
propanoyl-CoA + phosphate→propanoyl phosphate + coenzyme A (EC 2.3.1.222)
HEPCGN_14165
HEPCGN_21995 (pta)
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
HEPCGN_13350 (ilvD)
Acetate kinase (in reverse):
propanoyl phosphate + ADP→propanoate + ATP (EC 2.7.2.1; 2.7.2.14; 2.7.2.15; 2.7.2.7)
HEPCGN_17245 (tdcD)
HEPCGN_22000 (ackA)
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
HEPCGN_13355 (ilvE)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information