MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Escherichia coli ECOR38

Add experiment(s):


L-lactate dehydrogenase:
(S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
HEPCGN_07625 (ykgF)
HEPCGN_07630 (ykgE)
HEPCGN_10705
HEPCGN_10710
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
HEPCGN_01895 (ybiW)
HEPCGN_01900 (ybiY)
HEPCGN_12260 (pflC)
HEPCGN_12265 (pflD)
HEPCGN_14175 (cutD)
HEPCGN_14180 (cutC)
HEPCGN_17250 (tdcE)
HEPCGN_20470 (grcA)
HEPCGN_23005 (pflA)
HEPCGN_23010 (pflB)
formate + H+→H2 + CO2
HEPCGN_11400 (fdhF)
HEPCGN_19765 (hycB)
HEPCGN_19770 (hycC)
HEPCGN_19775 (hycD)
HEPCGN_21060 (hyfC)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
HEPCGN_01775 (hcxB)
HEPCGN_16660 (mdh)
HEPCGN_26435 (maeA)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
HEPCGN_14165
HEPCGN_21200 (pta)
HEPCGN_21995 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
HEPCGN_04755 (purT)
HEPCGN_17245 (tdcD)
HEPCGN_21185 (eutP)
HEPCGN_21190 (eutQ)
HEPCGN_22000 (ackA)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
HEPCGN_01375 (ttdA)
HEPCGN_05680 (fumD)
HEPCGN_06020 (fumB)
HEPCGN_06025 (fumC)
HEPCGN_11160 (fumB)
HEPCGN_18660 (fumE)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
HEPCGN_10570 (frdA)
HEPCGN_10575 (frdB)
HEPCGN_10580
HEPCGN_10585 (frdD)
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
HEPCGN_18730 (scpC)
Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
HEPCGN_18745 (scpA)
Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information