MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Escherichia coli ECOR38

Add experiment(s):


Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
HEPCGN_14650 (mtlD)
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
HEPCGN_11830 (fabG)
HEPCGN_19870 (srlD)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
HEPCGN_01595 (ybhA)
HEPCGN_07265 (phoA)
HEPCGN_10145 (fbp)
HEPCGN_12425 (glpX)
HEPCGN_18655 (glpX)
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
HEPCGN_05400 (pfkB)
HEPCGN_12475 (pfkA)
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
HEPCGN_02790 (fbaB)
HEPCGN_14330
HEPCGN_14335 (fba)
HEPCGN_15410 (fba)
HEPCGN_18705 (fbaA)
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
HEPCGN_12455 (tpiA)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
HEPCGN_05120 (gapA)
HEPCGN_26125 (gap)
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
HEPCGN_18700 (pgk)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
HEPCGN_01540 (gpmA)
HEPCGN_09160
HEPCGN_14575 (gpmM)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
HEPCGN_01540 (gpmA)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
HEPCGN_19525 (eno)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
HEPCGN_04725 (pyk)
HEPCGN_05675 (pykF)
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
HEPCGN_07625 (ykgF)
HEPCGN_07630 (ykgE)
HEPCGN_10705
HEPCGN_10710
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
HEPCGN_05510 (ppsA)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
HEPCGN_01895 (ybiW)
HEPCGN_01900 (ybiY)
HEPCGN_12260 (pflC)
HEPCGN_12265 (pflD)
HEPCGN_14175 (cutD)
HEPCGN_14180 (cutC)
HEPCGN_17250 (tdcE)
HEPCGN_20470 (grcA)
HEPCGN_23005 (pflA)
HEPCGN_23010 (pflB)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
HEPCGN_07390 (mhpF)
HEPCGN_14195 (adhE)
HEPCGN_21215 (eutE)
HEPCGN_25070 (adhE)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
HEPCGN_14165
HEPCGN_21200 (pta)
HEPCGN_21995 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
HEPCGN_04755 (purT)
HEPCGN_17245 (tdcD)
HEPCGN_21185 (eutP)
HEPCGN_21190 (eutQ)
HEPCGN_22000 (ackA)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
HEPCGN_07365 (frmA)
HEPCGN_07515 (yahK)
HEPCGN_09935 (ahr)
HEPCGN_14690 (yiaY)
HEPCGN_16580 (acuI)
HEPCGN_25070 (adhE)
HEPCGN_26430 (adhP)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information