MetaCyc Pathway: heterolactic fermentation in Escherichia coli ECOR38

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Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
HEPCGN_07205 (mak)
HEPCGN_12655 (yihV)
HEPCGN_14340
HEPCGN_16555
HEPCGN_19180
Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
HEPCGN_07205 (mak)
HEPCGN_21530 (glk)
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
HEPCGN_11685 (pgi)
HEPCGN_19590 (kduI)
Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP+→6-phospho D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.49)
HEPCGN_04740 (zwf)
6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H2O→D-gluconate 6-phosphate + H+ (EC 3.1.1.31)
HEPCGN_01600 (pgl)
D-gluconate 6-phosphate + NAD(P)+→CO2 + D-ribulose 5-phosphate + NAD(P)H (EC 1.1.1.351)
No genes
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
HEPCGN_15950 (rpe)
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
HEPCGN_05120 (gapA)
HEPCGN_26125 (gap)
Phosphate acetyltransferase (in reverse):
acetyl phosphate + coenzyme A→acetyl-CoA + phosphate (EC 2.3.1.8)
HEPCGN_14165
HEPCGN_21200 (pta)
HEPCGN_21995 (pta)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
HEPCGN_07390 (mhpF)
HEPCGN_14195 (adhE)
HEPCGN_21215 (eutE)
HEPCGN_25070 (adhE)
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
HEPCGN_18700 (pgk)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
HEPCGN_07365 (frmA)
HEPCGN_07515 (yahK)
HEPCGN_09935 (ahr)
HEPCGN_14690 (yiaY)
HEPCGN_16580 (acuI)
HEPCGN_25070 (adhE)
HEPCGN_26430 (adhP)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
HEPCGN_01540 (gpmA)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
HEPCGN_19525 (eno)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
HEPCGN_04725 (pyk)
HEPCGN_05675 (pykF)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
HEPCGN_02225 (dld)
HEPCGN_26050 (ldhA)
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
HEPCGN_07625 (ykgF)
HEPCGN_07630 (ykgE)
HEPCGN_10705
HEPCGN_10710

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information