MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Escherichia coli ECOR38

Add experiment(s):


Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
HEPCGN_13345 (ilvA)
HEPCGN_17235 (tdcB)
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HEPCGN_13345 (ilvA)
HEPCGN_17235 (tdcB)
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HEPCGN_10070 (ridA)
HEPCGN_13345 (ilvA)
HEPCGN_17235 (tdcB)
HEPCGN_17255 (tdcF)
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
HEPCGN_08675 (ilvN)
HEPCGN_08680 (ilvI)
HEPCGN_13360
HEPCGN_13365 (ilvG)
HEPCGN_13885
HEPCGN_13890 (ilvN)
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
HEPCGN_13335 (ilvC)
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
HEPCGN_13350 (ilvD)
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
HEPCGN_13355 (ilvE)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information