MetaCyc Pathway: mixed acid fermentation in Escherichia coli ECOR38

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
HEPCGN_12240 (ppc)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
HEPCGN_04725 (pyk)
HEPCGN_05675 (pykF)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
HEPCGN_02225 (dld)
HEPCGN_26050 (ldhA)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
HEPCGN_01775 (hcxB)
HEPCGN_16660 (mdh)
HEPCGN_26435 (maeA)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
HEPCGN_01895 (ybiW)
HEPCGN_01900 (ybiY)
HEPCGN_12260 (pflC)
HEPCGN_12265 (pflD)
HEPCGN_14175 (cutD)
HEPCGN_14180 (cutC)
HEPCGN_17250 (tdcE)
HEPCGN_20470 (grcA)
HEPCGN_23005 (pflA)
HEPCGN_23010 (pflB)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
HEPCGN_07390 (mhpF)
HEPCGN_14195 (adhE)
HEPCGN_21215 (eutE)
HEPCGN_25070 (adhE)
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
HEPCGN_01275 (gltA)
formate + H+→CO2 + H2
HEPCGN_11400 (fdhF)
HEPCGN_19765 (hycB)
HEPCGN_19770 (hycC)
HEPCGN_19775 (hycD)
HEPCGN_21060 (hyfC)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
HEPCGN_01375 (ttdA)
HEPCGN_05680 (fumD)
HEPCGN_06020 (fumB)
HEPCGN_06025 (fumC)
HEPCGN_11160 (fumB)
HEPCGN_18660 (fumE)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
HEPCGN_14165
HEPCGN_21200 (pta)
HEPCGN_21995 (pta)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
HEPCGN_04755 (purT)
HEPCGN_17245 (tdcD)
HEPCGN_21185 (eutP)
HEPCGN_21190 (eutQ)
HEPCGN_22000 (ackA)
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
HEPCGN_01620 (acnA)
HEPCGN_08460 (acnB)
HEPCGN_25625 (acnA)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
HEPCGN_07365 (frmA)
HEPCGN_07515 (yahK)
HEPCGN_09935 (ahr)
HEPCGN_14690 (yiaY)
HEPCGN_16580 (acuI)
HEPCGN_25070 (adhE)
HEPCGN_26430 (adhP)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
HEPCGN_10570 (frdA)
HEPCGN_10575 (frdB)
HEPCGN_10580
HEPCGN_10585 (frdD)
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
HEPCGN_01620 (acnA)
HEPCGN_08460 (acnB)
HEPCGN_25625 (acnA)
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
HEPCGN_24245 (icd)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information