MetaCyc Pathway: ammonia assimilation cycle III in Escherichia coli ECOR38

Glutamate synthase (NADPH): L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)

HEPCGN_01050 (asnB)

HEPCGN_03595 (ybtS)

HEPCGN_04950 (pabB)

HEPCGN_06705 (glsA)

HEPCGN_08925 (carB)

HEPCGN_08930 (carA)

HEPCGN_16050 (pabA)

HEPCGN_16755 (gltD)

HEPCGN_16760 (gltB3)

HEPCGN_18900 (ygfT)

HEPCGN_19520 (pyrG)

HEPCGN_20285 (ypjD)

HEPCGN_20905 (guaA)

HEPCGN_21150 (aegA)

HEPCGN_25530 (trpD)

HEPCGN_25535 (trpE)

HEPCGN_26645 (glsB)

Glutamate synthase (NADPH) (in reverse): ammonia + 2-oxoglutarate + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)

HEPCGN_16755 (gltD)

HEPCGN_16760 (gltB3)

HEPCGN_18900 (ygfT)

HEPCGN_20285 (ypjD)

HEPCGN_21150 (aegA)

Glutamate--ammonia ligase: L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)

HEPCGN_12730 (glnA)

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information