MetaCyc Pathway: superpathway of thiamine diphosphate biosynthesis I in Escherichia coli ECOR27

Add experiment(s):


1-deoxy-D-xylulose-5-phosphate synthase:
D-glyceraldehyde 3-phosphate + H+ + pyruvate→1-deoxy-D-xylulose 5-phosphate + CO2 (EC 2.2.1.7)
NOLOHH_01370 (dxs)
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + S-adenosyl-L-methionine→4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + carbon monoxide + 5'-deoxyadenosine + formate + L-methionine + 3 H+ (EC 4.1.99.17)
NOLOHH_05520 (thiC)
L-tyrosine + NADPH + S-adenosyl-L-methionine→2-iminoacetate + 5'-deoxyadenosine + 4-methylphenol + L-methionine + NADP+ + H+ (EC 4.1.99.19)
NOLOHH_05545 (thiH)
Cysteine desulfurase:
L-cysteine + a [ThiI sulfur-carrier protein]-L-cysteine→a [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
NOLOHH_11980 (csdE)
NOLOHH_11985 (csdA)
NOLOHH_13290 (iscS)
NOLOHH_17790 (sufS)
a [ThiS sulfur-carrier protein] + ATP + H+→a carboxy-adenylated-[ThiS sulfur-carrier protein] + diphosphate (EC 2.7.7.73)
NOLOHH_05530 (thiF)
Phosphomethylpyrimidine kinase:
4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP→4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP (EC 2.7.4.7)
NOLOHH_15415
a carboxy-adenylated-[ThiS sulfur-carrier protein] + a [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + 2 a reduced ferredoxin [iron-sulfur] cluster→a thiocarboxylated-[ThiS sulfur-carrier protein] + AMP + 2 an oxidized ferredoxin [iron-sulfur] cluster + a [ThiI sulfur-carrier protein]-L-cysteine
NOLOHH_01355 (thiI)
2-iminoacetate + 1-deoxy-D-xylulose 5-phosphate + a thiocarboxylated-[ThiS sulfur-carrier protein]→a [ThiS sulfur-carrier protein] + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 H2O (EC 2.8.1.10)
NOLOHH_05540 (thiG)
Thiamine-phosphate diphosphorylase:
4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 H+→thiamine phosphate + CO2 + diphosphate (EC 2.5.1.3)
NOLOHH_05525 (thiE)
Thiamine-phosphate kinase:
thiamine phosphate + ATP→thiamine diphosphate + ADP (EC 2.7.4.16)
NOLOHH_01385 (thiL)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information