MetaCyc Pathway: lipid A-core biosynthesis (Salmonella) in Escherichia coli ECOR27

Add experiment(s):


ADP-L-glycero-β-D-manno-heptose + α-D-Kdo-(2→4)-α-D-Kdo-(2→6)-lipid A (E. coli)→α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.4.99.23)
NOLOHH_07570 (rfaC)
ADP-L-glycero-β-D-manno-heptose + α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli)→α-Hep-(1→3)-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.4.99.24)
NOLOHH_07575 (rfaF)
α-Hep-(1→3)-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ATP→α-Hep-(1→3)-4-O-phospho-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.7.1.235)
NOLOHH_07540 (rfaP)
ADP-L-glycero-β-D-manno-heptose + α-Hep-(1→3)-4-O-phospho-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli)→(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + ADP + H+ (EC 2.4.99.25)
NOLOHH_07530 (rfaQ)
(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + UDP-α-D-glucose→glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + H+ + UDP (EC 2.4.1.M73)
NOLOHH_07535 (rfaG)
glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + ATP→glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + ADP + H+
NOLOHH_07550 (rfaY)
glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + UDP-α-D-galactose→galactosyl-(1→6)-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP
No genes
Lipopolysaccharide 3-alpha-galactosyltransferase:
UDP-α-D-galactose + galactosyl-(1→6)-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→galactosyl-(1→3)-[galactosyl-(1→6)]-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate + H+ + UDP (EC 2.4.1.44)
NOLOHH_07545 (waaO)
UDP-α-D-galactose + galactosyl-(1→3)-[galactosyl-(1→6)]-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate→galactosyl2-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate (Salmonella typhimurium) + H+ + UDP
NOLOHH_07555 (rfaJ)
Lipopolysaccharide N-acetylglucosaminyltransferase:
galactosyl2-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate (Salmonella typhimurium) + UDP-N-acetyl-α-D-glucosamine→lipid A-core oligosaccharide (Salmonella typhimurium) + H+ + UDP (EC 2.4.1.56)
NOLOHH_07560 (rfaB)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information