MetaCyc Pathway: γ-glutamyl cycle in Escherichia coli ECOR27

Add experiment(s):


5-oxoprolinase (ATP-hydrolyzing):
5-oxo-L-proline + ATP + 2 H2O→L-glutamate + ADP + H+ + phosphate (EC 3.5.2.9)
NOLOHH_23380 (pxpA)
NOLOHH_23385 (pxpC)
NOLOHH_23390 (pxpB)
Glutamate--cysteine ligase:
L-glutamate + L-cysteine + ATP→γ-L-glutamyl-L-cysteine + ADP + H+ + phosphate (EC 6.3.2.2)
NOLOHH_12600 (gshA)
NOLOHH_24035 (ybdK)
Glutathione synthase:
γ-L-glutamyl-L-cysteine + ATP + glycine→glutathione + ADP + H+ + phosphate (EC 6.3.2.3)
NOLOHH_11145 (gshB)
Gamma-glutamyltransferase:
glutathione + a proteinogenic amino acid→an α-(γ-L-glutamyl)-L-amino acid + L-cysteinylglycine (EC 2.3.2.2)
NOLOHH_08590 (ggt)
an α-(γ-L-glutamyl)-L-amino acid→5-oxo-L-proline + an L-amino acid (EC 4.3.2.9)
No genes
Cytosol nonspecific dipeptidase:
L-cysteinylglycine + H2O→L-cysteine + glycine (EC 3.4.13.18)
NOLOHH_02075 (pepD)
NOLOHH_04055 (pepA)
NOLOHH_13325 (pepB)
NOLOHH_14025 (ypdF)
NOLOHH_21920 (pepN)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information