MetaCyc Pathway: superpathway of (Kdo)2-lipid A biosynthesis in Escherichia coli ECOR27

Add experiment(s):


Arabinose-5-phosphate isomerase (in reverse):
D-ribulose 5-phosphate→aldehydo-D-arabinose 5-phosphate (EC 5.3.1.13)
NOLOHH_09765 (kdsD)
NOLOHH_12500 (gutQ)
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase:
a (3R)-3-hydroxytetradecanoyl-[acp] + UDP-N-acetyl-α-D-glucosamine→UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-α-D-glucosamine + a soluble [acyl-carrier protein] (EC 2.3.1.129)
NOLOHH_02495 (lpxA)
aldehydo-D-arabinose 5-phosphate→D-arabinofuranose 5-phosphate (spontaneous)
No genes
UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-α-D-glucosamine + H2O→UDP-3-O-[(3R)-3-hydroxymyristoyl]-α-D-glucosamine + acetate (EC 3.5.1.108)
NOLOHH_02945 (lpxC)
3-deoxy-8-phosphooctulonate synthase:
D-arabinofuranose 5-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-manno-octulosonate 8-phosphate + phosphate (EC 2.5.1.55)
NOLOHH_20590 (kdsA)
a (3R)-3-hydroxytetradecanoyl-[acp] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-α-D-glucosamine→UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine + a soluble [acyl-carrier protein] + H+ (EC 2.3.1.191)
NOLOHH_02505 (lpxD)
3-deoxy-manno-octulosonate-8-phosphatase:
3-deoxy-D-manno-octulosonate 8-phosphate + H2O→3-deoxy-α-D-manno-2-octulosonate + phosphate (EC 3.1.3.45)
NOLOHH_09760 (kdsC)
UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine + H2O→lipid X (E. coli) + 2 H+ + UMP (EC 3.6.1.54)
NOLOHH_00820 (lpxH)
3-deoxy-manno-octulosonate cytidylyltransferase:
3-deoxy-α-D-manno-2-octulosonate + CTP→CMP-3-deoxy-β-D-manno-octulosonate + diphosphate (EC 2.7.7.38)
NOLOHH_21995 (kdsB)
Lipid-A-disaccharide synthase:
lipid X (E. coli) + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine→lipid A disaccharide (E. coli) + H+ + UDP (EC 2.4.1.182)
NOLOHH_02490 (lpxB)
Tetraacyldisaccharide 4'-kinase:
lipid A disaccharide (E. coli) + ATP→lipid IVA (E. coli) + ADP + H+ (EC 2.7.1.130)
NOLOHH_22010 (lpxK)
CMP-3-deoxy-β-D-manno-octulosonate + lipid IVA (E. coli)→α-Kdo-(2→6)-lipid IVA (E. coli) + CMP + H+ (EC 2.4.99.12)
NOLOHH_07525 (waaA)
CMP-3-deoxy-β-D-manno-octulosonate + α-Kdo-(2→6)-lipid IVA (E. coli)→α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H+ (EC 2.4.99.13)
NOLOHH_07525 (waaA)
α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA (E. coli) + a dodecanoyl-[acp]→α-Kdo-(2->4)-α-Kdo-(2->6)-(lauroyl)-lipid IVA (E. coli) + a soluble [acyl-carrier protein] (EC 2.3.1.241)
NOLOHH_21300 (lpxL)
α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA (E. coli) + a palmitoleoyl-[acp]→Kdo2-(palmitoleoyl)-lipid IVA + a soluble [acyl-carrier protein] (EC 2.3.1.242)
NOLOHH_14065 (lpxP)
α-Kdo-(2->4)-α-Kdo-(2->6)-(lauroyl)-lipid IVA (E. coli) + a myristoyl-[acp]→α-D-Kdo-(2→4)-α-D-Kdo-(2→6)-lipid A (E. coli) + a soluble [acyl-carrier protein] (EC 2.3.1.243)
NOLOHH_16755 (lpxM)
Kdo2-(palmitoleoyl)-lipid IVA + a myristoyl-[acp]→(Kdo)2-lipid A, cold adapted + a soluble [acyl-carrier protein] (EC 2.3.1.243)
NOLOHH_16755 (lpxM)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information