MetaCyc Pathway: mixed acid fermentation in Escherichia coli ECOR27

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
NOLOHH_05740 (ppc)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
NOLOHH_16835 (pyk)
NOLOHH_17810 (pykF)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
NOLOHH_15190 (dld)
NOLOHH_19530 (ldhA)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
NOLOHH_09595 (mdh)
NOLOHH_19035 (maeA)
NOLOHH_22620 (hcxB)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
NOLOHH_05760 (pflC)
NOLOHH_05765 (pflD)
NOLOHH_10195 (tdcE)
NOLOHH_13015 (grcA)
NOLOHH_22070 (pflB)
NOLOHH_22075 (pflA)
NOLOHH_22485 (ybiY)
NOLOHH_22490 (ybiW)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
NOLOHH_01680 (mhpF)
NOLOHH_13725 (eutE)
NOLOHH_20445 (adhE)
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
NOLOHH_23345 (gltA)
formate + H+→CO2 + H2
NOLOHH_05025 (fdhF)
NOLOHH_12420 (hycB)
NOLOHH_12425 (hycC)
NOLOHH_12430 (hycD)
NOLOHH_13550 (hyfC)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
NOLOHH_04790 (fumB)
NOLOHH_11260 (fumE)
NOLOHH_17815 (fumD)
NOLOHH_18150 (fumB)
NOLOHH_18155 (fumC)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
NOLOHH_13710 (pta)
NOLOHH_14410 (pta)
NOLOHH_15965
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
NOLOHH_10190 (tdcD)
NOLOHH_13695 (eutP)
NOLOHH_13700 (eutQ)
NOLOHH_14415 (ackA)
NOLOHH_16860 (purT)
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
NOLOHH_02825 (acnB)
NOLOHH_20240 (acnA)
NOLOHH_23085 (ybhJ)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
NOLOHH_01650 (frmA)
NOLOHH_01805 (yahK)
NOLOHH_04010 (ahr)
NOLOHH_07750 (yiaY)
NOLOHH_09500 (acuI)
NOLOHH_19040 (adhP)
NOLOHH_20445 (adhE)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
NOLOHH_04605 (frdA)
NOLOHH_04610 (frdB)
NOLOHH_04615
NOLOHH_04620 (frdD)
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
NOLOHH_02825 (acnB)
NOLOHH_20240 (acnA)
NOLOHH_23085 (ybhJ)
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
NOLOHH_20885 (icd)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information