MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Dickeya dianthicola ME23

MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Dickeya dianthicola ME23

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

Sulfate adenylyltransferase:
sulfate + ATP + H⁺→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)

Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H⁺ (EC 2.7.1.25)

Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)

Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H⁺ (EC 1.8.4.8)

Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H⁺→L-aspartate 4-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.11)

Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H⁺→L-homoserine + NAD(P)⁺ (EC 1.1.1.3)

Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H⁺→hydrogen sulfide + 3 NADP⁺ + 3 H₂O (EC 1.8.1.2)

Homoserine O-acetyltransferase:
L-homoserine + acetyl-CoA→O-acetyl-L-homoserine + coenzyme A (EC 2.3.1.31)

O-acetylhomoserine aminocarboxypropyltransferase:
O-acetyl-L-homoserine + hydrogen sulfide→L-homocysteine + acetate + H⁺ (EC 2.5.1.49)

5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)

Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)

Links:

Pathway details at MetaCyc
Fitness data for 24 genes
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All MetaCyc pathways for Dickeya dianthicola ME23

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information