MetaCyc Pathway: (5Z)-dodecenoate biosynthesis I in Dickeya dianthicola 67-19

MetaCyc Pathway: (5Z)-dodecenoate biosynthesis I in Dickeya dianthicola 67-19

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp] (EC 5.3.3.14)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H⁺→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H⁺→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H⁺→a (5Z)-dodec-5-enoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Links:

Pathway details at MetaCyc
Fitness data for 17 genes
Select organism:
All MetaCyc pathways for Dickeya dianthicola 67-19

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information