MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Dickeya dadantii 3937

Aspartate transaminase (in reverse): oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

DDA3937_RS00500

DDA3937_RS01120

DDA3937_RS02130

DDA3937_RS07555

DDA3937_RS08230

DDA3937_RS12915

DDA3937_RS14050

DDA3937_RS14180

DDA3937_RS14345

Aspartate kinase: L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)

DDA3937_RS00955

DDA3937_RS18415

DDA3937_RS19020

Aspartate-semialdehyde dehydrogenase (in reverse): L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)

DDA3937_RS14470

DDA3937_RS19765

Homoserine dehydrogenase (in reverse): L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)

DDA3937_RS00955

DDA3937_RS18415

Homoserine kinase: L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)

DDA3937_RS18410

Threonine synthase: O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)

DDA3937_RS18405

Threonine ammonia-lyase: L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)

DDA3937_RS08075

DDA3937_RS20150

1-aminocyclopropane-1-carboxylate deaminase: (Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

DDA3937_RS02260

DDA3937_RS08075

DDA3937_RS20150

2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

DDA3937_RS01915

DDA3937_RS02260

DDA3937_RS08075

DDA3937_RS20150

Acetolactate synthase: 2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)

DDA3937_RS03235

DDA3937_RS18100

DDA3937_RS18105

DDA3937_RS18170

DDA3937_RS18175

DDA3937_RS20165

DDA3937_RS20170

Ketol-acid reductoisomerase (in reverse): (S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)

DDA3937_RS20130

Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)

DDA3937_RS20155

Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

DDA3937_RS11775

DDA3937_RS20160

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information