MetaCyc Pathway: glutaminyl-tRNAgln biosynthesis via transamidation in Brevundimonas sp. GW460-12-10-14-LB2

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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
A4249_RS00725
A4249_RS01055
A4249_RS02530
A4249_RS03045
A4249_RS04280
A4249_RS04285
A4249_RS04290
A4249_RS06135
A4249_RS06155
A4249_RS06165
A4249_RS12055
A4249_RS16105
A4249_RS16110
A4249_RS16140
Glutamate--tRNA(Gln) ligase:
L-glutamate + ATP + a tRNAGln→an L-glutamyl-[tRNAGln] + AMP + diphosphate (EC 6.1.1.24)
A4249_RS08525
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
an L-glutamyl-[tRNAGln] + ATP→a 5-phosphooxy-L-glutamyl-[tRNAGln] + ADP (EC 6.3.5.7)
A4249_RS04280
A4249_RS04285
A4249_RS04290
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
a 5-phosphooxy-L-glutamyl-[tRNAGln] + ammonia→an L-glutaminyl-[tRNAGln] + phosphate (EC 6.3.5.7)
A4249_RS04280
A4249_RS04285
A4249_RS04290

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information