MetaCyc Pathway: UMP biosynthesis II in Bosea sp. OAE506

MetaCyc Pathway: UMP biosynthesis II in Bosea sp. OAE506

Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + 2 ATP + hydrogen carbonate + H₂O→carbamoyl phosphate + 2 ADP + L-glutamate + 2 H⁺ + phosphate (EC 6.3.5.5)

Aspartate carbamoyltransferase:
carbamoyl phosphate + L-aspartate→N-carbamoyl-L-aspartate + H⁺ + phosphate (EC 2.1.3.2)

Dihydroorotase (in reverse):
N-carbamoyl-L-aspartate + H⁺→(S)-dihydroorotate + H₂O (EC 3.5.2.3)

(S)-dihydroorotate + fumarate→orotate + succinate (EC 1.3.98.1)

Orotate phosphoribosyltransferase (in reverse):
orotate + 5-phospho-α-D-ribose 1-diphosphate→orotidine 5'-phosphate + diphosphate (EC 2.4.2.10)

Orotidine-5'-phosphate decarboxylase:
orotidine 5'-phosphate + H⁺→UMP + CO₂ (EC 4.1.1.23)

Links:

Pathway details at MetaCyc
Fitness data for 11 genes
Select organism:
All MetaCyc pathways for Bosea sp. OAE506

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information