| malonyl-CoA + a holo ACP domain within [AprA PKS]→malonyl-[ACP domain within AprA PKS] + coenzyme A |
| ABI39_RS20620 |
| L-cysteine + ATP + a holo-[non-ribosomal peptide synthase AprH]→L-cysteinyl-[non-ribosomal peptide synthase AprH] + AMP + diphosphate (EC 6.2.1.69) |
| No genes |
| L-tyrosine + ATP + a holo-PCPI domain within [AprJ NRPS]→tyrosyl-[PCPI domain within AprJ NRPS] + AMP + diphosphate (EC 6.2.1.M39) |
| No genes |
| L-alanine + ATP + a holo-PCPII domain within [AprJ NRPS]→L-alanyl-[PCPII domain within AprJ NRPS] + AMP + diphosphate (EC 6.2.1.67) |
| No genes |
| L-isoleucine + ATP + a holo-PCP domain within [AprK NRPS]→L-isoleucinyl-[PCP domain within AprK NRPS] + AMP + diphosphate |
| No genes |
| L-proline + ATP + a holo-PCP domain within [AprL NRPS]→L-prolyl-[PCP domain within AprLK NRPS] + AMP + diphosphate (EC 6.2.1.53) |
| No genes |
| malonyl-[ACP domain within AprA PKS] + 2 S-adenosyl-L-methionine→2,2-dimethylmalonyl-[ACP domain within AprA PKS] + 2 S-adenosyl-L-homocysteine + 2 H+ |
| No genes |
| 2,2-dimethylmalonyl-[ACP domain within AprA PKS] + S-adenosyl-L-methionine→pivaloyl-[ACP domain within AprA PKS] + S-adenosyl-L-homocysteine + CO2 |
| No genes |
| malonyl-CoA + pivaloyl-[ACP domain within AprA PKS] + a holo ACPI domain within [AprB PKS] + NADPH + 2 H+→(3S)-3-hydroxy-4,4-dimethylbutanoyl-[ACPI domain within AprB PKS] + a holo ACP domain within [AprA PKS] + CO2 + coenzyme A + NADP+ |
| No genes |
| (3S)-3-hydroxy-4,4-dimethylbutanoyl-[ACPI domain within AprB PKS] + malonyl-CoA + a holo ACPII domain within [AprB PKS] + H+→(5S)-5-hydroxy-6,6-dimethyl-3-oxoheptanoyl-[ACPII domain within AprB PKS] + a holo ACPI domain within [AprB PKS] + CO2 + coenzyme A |
| No genes |
| (5S)-5-hydroxy-6,6-dimethyl-3-oxoheptanoyl-[ACPII domain within AprB PKS] + a holo [AprC acyl-carrier protein]→(5S)-5-hydroxy-6,6-dimethyl-3-oxoheptanoyl-[AprC acyl-carrier protein] + a holo ACPII domain within [AprB PKS] |
| No genes |
| (5S)-5-hydroxy-6,6-dimethyl-3-oxoheptanoyl-[AprC acyl-carrier protein] + an acetyl-[acp] + H2O→(5S)-3-carboxymethyl-3,5-dihydroxy-6,6-dimethyl-3-acetyl-heptanoyl-[AprC acyl-carrier protein] + a soluble [acyl-carrier protein] + H+ (EC 2.3.3.g) |
| No genes |
| (5S)-3-carboxymethyl-3,5-dihydroxy-6,6-dimethyl-3-acetyl-heptanoyl-[AprC acyl-carrier protein]→(5S)-3-acetyl-6,6-dimethyl-5-hydroxy-hept-2-enoyl-[AprC acyl-carrier protein] + H2O (EC 4.2.1.ca) |
| No genes |
| (5S)-3-acetyl-6,6-dimethyl-5-hydroxy-hept-2-enoyl-[AprC acyl-carrier protein] + H+→(5S)-5-hydroxy-3,6,6-trimethylhept-2-enoyl-[AprC acyl-carrier protein] + CO2 (EC 4.1.1.ai) |
| No genes |
| (5S)-5-hydroxy-3,6,6-trimethylhept-2-enoyl-[AprC acyl-carrier protein] + malonyl-CoA + a holo ACP domain within [AprG PKS] + 2 NADPH + 2 H+ + S-adenosyl-L-methionine→(2S,3S,5S,7S)-3,7-dihydroxy-2,5,8,8-tetramethylnonanoyl-[ACP domain within AprG PKS] + S-adenosyl-L-homocysteine + a holo [AprC acyl-carrier protein] + CO2 + coenzyme A + 2 NADP+ |
| No genes |
| (2S,3S,5S,7S)-3,7-dihydroxy-2,5,8,8-tetramethylnonanoyl-[ACP domain within AprG PKS] + L-cysteinyl-[non-ribosomal peptide synthase AprH]→aparatoxin intermediate-[non-ribosomal peptide synthase AprH] + a holo ACP domain within [AprG PKS] + H+ + H2O |
| No genes |
| aparatoxin intermediate-[non-ribosomal peptide synthase AprH] + malonyl-CoA + a holo ACP domain within [AprI PKS] + NADPH + H+ + S-adenosyl-L-methionine→apratoxin intermediate-[AprI PKS] + S-adenosyl-L-homocysteine + CO2 + coenzyme A + NADP+ + H2O + a holo-[non-ribosomal peptide synthase AprH] |
| No genes |
| apratoxin intermediate-[AprI PKS] + tyrosyl-[PCPI domain within AprJ NRPS] + S-adenosyl-L-methionine→apratoxin intermediate-[PCPI domain within AprJ NRPS] + S-adenosyl-L-homocysteine + a holo ACP domain within [AprI PKS] + 2 H+ |
| No genes |
| L-alanyl-[PCPII domain within AprJ NRPS] + apratoxin intermediate-[PCPI domain within AprJ NRPS] + S-adenosyl-L-methionine→apratoxin intermediate-[PCPII domain within AprJ NRPS] + S-adenosyl-L-homocysteine + a holo-PCPI domain within [AprJ NRPS] + 2 H+ |
| No genes |
| apratoxin intermediate-[PCPII domain within AprJ NRPS] + L-isoleucinyl-[PCP domain within AprK NRPS] + S-adenosyl-L-methionine→apratoxin intermediate-[PCP domain within AprK NRPS] + S-adenosyl-L-homocysteine + a holo-PCPII domain within [AprJ NRPS] + 2 H+ |
| No genes |
| apratoxin intermediate-[PCP domain within AprK NRPS] + L-prolyl-[PCP domain within AprLK NRPS]→apratoxin intermediate-[PCP domain within AprL NRPS] + a holo-PCP domain within [AprK NRPS] + H+ |
| No genes |
| apratoxin intermediate-[PCP domain within AprL NRPS]→apratoxin A + a holo-PCP domain within [AprL NRPS] |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information