MetaCyc Pathway: gondoate biosynthesis (anaerobic) in Escherichia coli BL21

Beta-ketoacyl-acyl-carrier-protein synthase II:
an oleoyl-[acp] + a malonyl-[acp] + H⁺→an (11Z)-3-oxo-icos-11-enoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

ECD_01091

ECD_02248

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (11Z)-3-oxo-icos-11-enoyl-[acp]_[cytosol] + NADPH_[cytosol] + H⁺_[cytosol]→a (3R,11Z)-3-hydroxy-icos-11-enoyl-[acp]_[cytosol] + NADP⁺_[cytosol] (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,11Z)-3-hydroxy-icos-11-enoyl-[acp]_[cytosol]→a (2E,11Z)-icosa-2,11-dienoyl-[acp]_[cytosol] + H₂O_[cytosol] (EC 4.2.1.59)

ECD_00178

ECD_00958

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,11Z)-icosa-2,11-dienoyl-[acp]_[cytosol] + NADH_[cytosol] + H⁺_[cytosol]→a gondoyl-[acp]_[cytosol] + NAD⁺_[cytosol] (EC 1.3.1.9)

ECD_01265

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Pathway details at MetaCyc
Fitness data for 9 genes
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All MetaCyc pathways for Escherichia coli BL21

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information