MetaCyc Pathway: acetyl-CoA fermentation to butanoate in Escherichia coli BL21

MetaCyc Pathway: acetyl-CoA fermentation to butanoate in Escherichia coli BL21

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)

Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)

Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H⁺→(3R)-3-hydroxybutanoyl-CoA + NADP⁺ (EC 1.1.1.36)

No genes

3-hydroxybutyryl-CoA dehydratase:
(3R)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.55)

crotonyl-CoA_[cytosol] + 2 NADH_[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster_[cytosol]→butanoyl-CoA_[cytosol] + 2 NAD⁺_[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster_[cytosol] (EC 1.3.1.109)

No genes

Propionate CoA-transferase:
acetate + butanoyl-CoA→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)

Links:

Pathway details at MetaCyc
Fitness data for 16 genes
Select organism:
All MetaCyc pathways for Escherichia coli BL21

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information