MetaCyc Pathway: γ-glutamyl cycle in Escherichia coli BL21

MetaCyc Pathway: γ-glutamyl cycle in Escherichia coli BL21

5-oxoprolinase (ATP-hydrolyzing):
5-oxo-L-proline + ATP + 2 H₂O→L-glutamate + ADP + H⁺ + phosphate (EC 3.5.2.9)

Glutamate--cysteine ligase:
L-glutamate + L-cysteine + ATP→γ-L-glutamyl-L-cysteine + ADP + H⁺ + phosphate (EC 6.3.2.2)

Glutathione synthase:
γ-L-glutamyl-L-cysteine + ATP + glycine→glutathione + ADP + H⁺ + phosphate (EC 6.3.2.3)

Gamma-glutamyltransferase:
glutathione + a proteinogenic amino acid→an α-(γ-L-glutamyl)-L-amino acid + L-cysteinylglycine (EC 2.3.2.2)

an α-(γ-L-glutamyl)-L-amino acid→5-oxo-L-proline + an L-amino acid (EC 4.3.2.9)

No genes

Cytosol nonspecific dipeptidase:
L-cysteinylglycine + H₂O→L-cysteine + glycine (EC 3.4.13.18)

Links:

Pathway details at MetaCyc
Fitness data for 12 genes
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All MetaCyc pathways for Escherichia coli BL21

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information