MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Escherichia coli BL21

MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Escherichia coli BL21

Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H₂O (EC 4.3.1.19)

1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

2-iminobutanoate + H₂O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

Acetolactate synthase:
2-oxobutanoate + H⁺ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO₂ (EC 2.2.1.6)

Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H⁺→(R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ (EC 1.1.1.86)

Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H₂O (EC 4.2.1.9)

Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

Links:

Pathway details at MetaCyc
Fitness data for 13 genes
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All MetaCyc pathways for Escherichia coli BL21

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information