MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Dickeya dadantii 3937

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Reactions and Genes M9+glycerol
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
DDA3937_RS00500 -0.3
DDA3937_RS01120 +0.1
DDA3937_RS02130 +0.1
DDA3937_RS07555 -0.1
DDA3937_RS08230 +0.1
DDA3937_RS12915 -2.1
DDA3937_RS14050 -0.1
DDA3937_RS14180 +0.3
DDA3937_RS14345 +0.7
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
DDA3937_RS00955 +0.2
DDA3937_RS18415 -2.7
DDA3937_RS19020 -0.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
DDA3937_RS14470 +0.5
DDA3937_RS19765 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
DDA3937_RS00955 +0.2
DDA3937_RS18415 -2.7
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
DDA3937_RS18410 -1.4
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
DDA3937_RS18405 -3.1
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
DDA3937_RS08075 +0.2
DDA3937_RS20150 -2.8
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
DDA3937_RS02260 +0.4
DDA3937_RS08075 +0.2
DDA3937_RS20150 -2.8
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
DDA3937_RS01915 -0.6
DDA3937_RS02260 +0.4
DDA3937_RS08075 +0.2
DDA3937_RS20150 -2.8
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
DDA3937_RS03235 +0.0
DDA3937_RS18100 +0.1
DDA3937_RS18105 -0.0
DDA3937_RS18170 -1.5
DDA3937_RS18175 -0.5
DDA3937_RS20165 -0.3
DDA3937_RS20170 -0.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
DDA3937_RS20130 -3.0
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
DDA3937_RS20155 -4.1
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
DDA3937_RS11775 -0.0
DDA3937_RS20160 -0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information