MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Dickeya dadantii 3937

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Reactions and Genes M9+glycerol
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
DDA3937_RS08075 +0.2
DDA3937_RS20150 -2.8
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
DDA3937_RS02260 +0.4
DDA3937_RS08075 +0.2
DDA3937_RS20150 -2.8
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
DDA3937_RS01915 -0.6
DDA3937_RS02260 +0.4
DDA3937_RS08075 +0.2
DDA3937_RS20150 -2.8
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
DDA3937_RS03235 +0.0
DDA3937_RS18100 +0.1
DDA3937_RS18105 -0.0
DDA3937_RS18170 -1.5
DDA3937_RS18175 -0.5
DDA3937_RS20165 -0.3
DDA3937_RS20170 -0.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
DDA3937_RS20130 -3.0
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
DDA3937_RS20155 -4.1
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
DDA3937_RS11775 -0.0
DDA3937_RS20160 -0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information