MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Escherichia coli BW25113

Add experiment(s):


Reactions and Genes LB Anaerobic with Cephalothin 0.0000078125 mM
remove
2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
b0074 (leuA) -0.2
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
b0071 (leuD) -0.1
b0072 (leuC) +0.2
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
b0071 (leuD) -0.1
b0072 (leuC) +0.2
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
b0073 (leuB) -0.4
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
b0073 (leuB) -0.4
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) -0.3
b0356 (frmA) -0.5
b1241 (adhE) -1.9
b1478 (adhP) +0.1
b3253 (yhdH) -1.5
b4269 (yjgB) -0.3
b3589 (yiaY) -0.1

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information