MetaCyc Pathway: superpathway of Clostridium acetobutylicum solventogenic fermentation in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Cephalothin 0.0000078125 mM
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
b1378 (ydbK) -0.0
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF) +0.1
b1241 (adhE) -1.9
b2455 (eutE) -0.0
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
b1397 (paaJ) -0.3
b2224 (atoB) -0.2
b2342 (yfcY) -0.8
b2844 (yqeF) +0.4
b3845 (fadA) +0.0
Butyrate--acetoacetate CoA-transferase (in reverse):
acetoacetyl-CoA + butanoate→acetoacetate + butanoyl-CoA (EC 2.8.3.9)
No genes
Butyrate--acetoacetate CoA-transferase (in reverse):
acetoacetyl-CoA + acetate→acetoacetate + acetyl-CoA (EC 2.8.3.9)
b2221 (atoD) -0.8
b2222 (atoA) -0.0
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) -0.3
b0356 (frmA) -0.5
b1241 (adhE) -1.9
b1478 (adhP) +0.1
b3253 (yhdH) -1.5
b4269 (yjgB) -0.3
b3589 (yiaY) -0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
b2341 (yfcX) -0.4
b3846 (fadB) -0.1
Acetoacetate decarboxylase:
acetoacetate + H+→acetone + CO2 (EC 4.1.1.4)
No genes
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
b2341 (yfcX) -0.4
b3846 (fadB) -0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information