| Reactions and Genes | LB Anaerobic with Cephalothin 0.0000078125 mM |
|---|
| remove |
Guanine deaminase:
guanine + H+ + H2O→xanthine + ammonium (EC 3.5.4.3) |
| b2883 (guaD) | +0.2 |
Xanthine dehydrogenase:
hypoxanthine + NAD+ + H2O→6,8-dihydroxypurine + NADH + H+ (EC 1.17.1.4) |
| b0284 (yagR) | -0.4 |
| b0285 (yagS) | +0.3 |
| b2866 (xdhA) | -0.1 |
| b2867 (xdhB) | -0.6 |
| b2868 (xdhC) | -0.1 |
Xanthine dehydrogenase:
purine + NAD+ + H2O→8-hydroxypurine + NADH + H+ (EC 1.17.1.4) |
| b0284 (yagR) | -0.4 |
| b0285 (yagS) | +0.3 |
| b2866 (xdhA) | -0.1 |
| b2867 (xdhB) | -0.6 |
| b2868 (xdhC) | -0.1 |
| 8-hydroxypurine + NAD+ + H2O→6,8-dihydroxypurine + NADH + H+ |
| No genes |
Xanthine dehydrogenase:
6,8-dihydroxypurine + NAD+ + H2O→urate + NADH + H+ (EC 1.17.1.4) |
| b0284 (yagR) | -0.4 |
| b0285 (yagS) | +0.3 |
| b2866 (xdhA) | -0.1 |
| b2867 (xdhB) | -0.6 |
| b2868 (xdhC) | -0.1 |
Xanthine dehydrogenase (in reverse):
urate + NADH + H+→xanthine + NAD+ + H2O (EC 1.17.1.4) |
| b0284 (yagR) | -0.4 |
| b0285 (yagS) | +0.3 |
| b2866 (xdhA) | -0.1 |
| b2867 (xdhB) | -0.6 |
| b2868 (xdhC) | -0.1 |
| xanthine + H2O→5-ureidoimidazole-4-carboxylate + H+ |
| No genes |
| 5-ureidoimidazole-4-carboxylate + 2 H+ + H2O→5-aminoimidazole-4-carboxylate + CO2 + ammonium |
| No genes |
| 5-aminoimidazole-4-carboxylate→CO2 + 4-aminoimidazole |
| No genes |
Aminoimidazolase:
4-aminoimidazole + H+ + H2O→3,5-dihydro-4H-imidazol-4-one + ammonium (EC 3.5.4.8) |
| No genes |
| 3,5-dihydro-4H-imidazol-4-one + H2O→N-formimino-glycine (spontaneous) |
| No genes |
Glycine formimidoyltransferase (in reverse):
N-formimino-glycine + a tetrahydrofolate→5-formimidoyltetrahydrofolate + glycine (EC 2.1.2.4) |
| No genes |
Formimidoyltetrahydrofolate cyclodeaminase:
5-formimidoyltetrahydrofolate + 2 H+→a 5,10-methenyltetrahydrofolate + ammonium (EC 4.3.1.4) |
| No genes |
Methenyltetrahydrofolate cyclohydrolase:
a 5,10-methenyltetrahydrofolate + H2O→an N10-formyltetrahydrofolate + H+ (EC 3.5.4.9) |
| b0529 (folD) | N.D. |
Methylenetetrahydrofolate dehydrogenase (NADP(+)) (in reverse):
a 5,10-methenyltetrahydrofolate + NADPH→a 5,10-methylenetetrahydrofolate + NADP+ (EC 1.5.1.5) |
| b0529 (folD) | N.D. |
Formyltetrahydrofolate deformylase:
an N10-formyltetrahydrofolate + H2O→formate + H+ + a tetrahydrofolate (EC 3.5.1.10) |
| b1232 (purU) | -0.0 |
Glycine hydroxymethyltransferase (in reverse):
glycine + a 5,10-methylenetetrahydrofolate + H2O→L-serine + a tetrahydrofolate (EC 2.1.2.1) |
| b2551 (glyA) | N.D. |
| formate + NAD+→CO2 + NADH (EC 1.17.1.9) |
| b1476 (fdnI) | -1.9 |
| b1499 (ydeO) | -0.1 |
| b1501 (YdeP) | +0.1 |
| b3892 (fdoI) | -0.0 |
| b3893 (fdoH) | -0.3 |
| b3895 (fdhD) | -0.1 |
L-serine ammonia-lyase:
L-serine→2-aminoprop-2-enoate + H2O (EC 4.3.1.17) |
| b1814 (sdaA) | -1.4 |
| b2797 (sdaB) | -1.1 |
| b4471 (tdcG) | -0.2 |
Tryptophanase:
2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2) |
| b1814 (sdaA) | -1.4 |
| b1919 (yedO) | +0.3 |
| b2366 (dsdA) | -1.5 |
| b2797 (sdaB) | -1.1 |
| b3708 (tnaA) | +0.1 |
| b4471 (tdcG) | -0.2 |
| 2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2) |
| b1814 (sdaA) | -1.4 |
| b1919 (yedO) | +0.3 |
| b2366 (dsdA) | -1.5 |
| b2797 (sdaB) | -1.1 |
| b3113 (yhaR) | +0.1 |
| b3708 (tnaA) | +0.1 |
| b4243 (yjgF) | -1.0 |
| b4471 (tdcG) | -0.2 |
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→CO2 + acetyl-CoA + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1) |
| b1378 (ydbK) | -0.0 |
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8) |
| b2297 (pta) | -0.1 |
| b2458 (eutI) | -0.9 |
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15) |
| b1849 (purT) | -0.0 |
| b2296 (ackA) | N.D. |
| b2460 (eutQ) | -0.1 |
| b2461 (eutP) | -0.0 |
| b3115 (tdcD) | -0.5 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information