MetaCyc Pathway: mannitol cycle in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
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Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
b0394 (yajF) -0.2
b3883 (yihV) -0.1
Mannitol-1-phosphate 5-dehydrogenase (in reverse):
β-D-fructofuranose 6-phosphate + NADH + H+→D-mannitol 1-phosphate + NAD+ (EC 1.1.1.17)
b3600 (mtlD) -1.8
Mannitol-1-phosphatase:
D-mannitol 1-phosphate + H2O→D-mannitol + phosphate (EC 3.1.3.22)
b1727 (yniC) -0.9
b2293 (yfbT) +0.4
Mannitol 2-dehydrogenase:
D-mannitol + NAD+→keto-D-fructose + NADH + H+ (EC 1.1.1.67)
No genes
keto-D-fructose→β-D-fructofuranose (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information