MetaCyc Pathway: UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) in Escherichia coli BW25113

Reactions and Genes	LB Anaerobic with Mecillinam 0.00003125 mM
D-alanine--D-alanine ligase: 2 D-alanine + ATP→D-alanyl-D-alanine + ADP + H+ + phosphate (EC 6.3.2.4)
b0092 (ddlB)	+0.2
b0381 (ddlA)	-1.9
Glutamate racemase: L-glutamate→D-glutamate (EC 5.1.1.3)
b3967 (murI)	N.D.
UDP-N-acetylglucosamine 1-carboxyvinyltransferase: UDP-N-acetyl-α-D-glucosamine + phosphoenolpyruvate→UDP-N-acetyl-α-D-glucosamine-enolpyruvate + phosphate (EC 2.5.1.7)
b3189 (murA)	N.D.
UDP-N-acetyl-α-D-glucosamine-enolpyruvate + NADPH + H+→UDP-N-acetyl-α-D-muramate + NADP+ (EC 1.3.1.98)
b3972 (murB)	N.D.
UDP-N-acetylmuramate--L-alanine ligase: UDP-N-acetyl-α-D-muramate + L-alanine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanine + ADP + H+ + phosphate (EC 6.3.2.8)
b0091 (murC)	N.D.
UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase: UDP-N-acetyl-α-D-muramoyl-L-alanine + D-glutamate + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + ADP + H+ + phosphate (EC 6.3.2.9)
b0088 (murD)	-0.8
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase: UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + meso-diaminopimelate + ATP→UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP + ADP + H+ + phosphate (EC 6.3.2.13)
b0085 (murE)	N.D.
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase: D-alanyl-D-alanine + UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP + ATP→UDP-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala-D-Ala + ADP + H+ + phosphate (EC 6.3.2.10)
b0086 (murF)	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information