MetaCyc Pathway: heterolactic fermentation in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Furaltadone 0.000015625 mM
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Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
b0394 (yajF) -0.2
b3883 (yihV) -0.2
Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
b0394 (yajF) -0.2
b2388 (glk) +0.3
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
b2843 (kduI) -0.0
b4025 (pgi) -2.7
Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP+→6-phospho D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.49)
b1852 (zwf) N.D.
6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H2O→D-gluconate 6-phosphate + H+ (EC 3.1.1.31)
b0767 (ybhE) +1.2
D-gluconate 6-phosphate + NAD(P)+→CO2 + D-ribulose 5-phosphate + NAD(P)H (EC 1.1.1.351)
No genes
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
b3386 (rpe) +0.5
b4301 (sgcE) -0.5
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA) N.D.
Phosphate acetyltransferase (in reverse):
acetyl phosphate + coenzyme A→acetyl-CoA + phosphate (EC 2.3.1.8)
b2297 (pta) -0.1
b2458 (eutI) +0.1
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF) -0.1
b1241 (adhE) -0.6
b2455 (eutE) +0.4
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk) N.D.
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) +0.2
b0356 (frmA) -0.1
b1241 (adhE) -0.6
b1478 (adhP) -0.4
b3253 (yhdH) -4.6
b4269 (yjgB) +0.3
b3589 (yiaY) +0.1
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
b0755 (gpmA) -0.1
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno) N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) -1.9
b1854 (pykA) +0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
b1380 (ldhA) -0.1
b2133 (dld) -0.2
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
b0306 (ykgE) -0.1
b0307 (ykgF) -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information