MetaCyc Pathway: NiFe(CO)(CN)2 cofactor biosynthesis in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Chloramphenicol 0.000001953125 mM
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[HypC dimer]-Fe-CO2 + a HypD [NiFe]-hydrogenase maturation protein→a [HypC/HypD dimer]-Fe-CO2 + a HypC [NiFe]-hydrogenase maturation protein
b2729 (hypD) -1.5
Ni2+ + GTP + a HypB hydrogenase accessory protein + H2O→Ni-[HypB hydrogenase accessory protein] + GDP + H+ + phosphate
b3349 (slyD) -1.0
carbamoyl phosphate + ATP + a HypE [NiFe]-hydrogenase maturation protein + H2O→a [HypE hydrogenase maturation protein]-S-carbamoyl-L-cysteine + AMP + diphosphate + H+ + phosphate
b2712 (hypF) -1.1
a [HypE hydrogenase maturation protein]-S-carbamoyl-L-cysteine + ATP→a [HypE hydrogenase maturation protein]-S-cyanyl-L-cysteine + ADP + H+ + phosphate (EC 4.2.1.M8)
b2730 (hypE) -1.1
a [HypC/HypD dimer]-Fe-CO2 + ATP + 2 a reduced one electron carrier→[HypC/HypD dimer]-Fe-CO + ADP + 2 an oxidized electron carrier + H+ + phosphate
b2729 (hypD) -1.5
Ni-[HypB hydrogenase accessory protein] + a HypA hydrogenase accessory protein→Ni-[HypA hydrogenase accessory protein] + a HypB hydrogenase accessory protein
b2727 (hypB) -1.2
[HypC/HypD dimer]-Fe-CO + 2 a [HypE hydrogenase maturation protein]-S-cyanyl-L-cysteine→[HypCDE]-Fe(CO)(CN)2
b2730 (hypE) -1.1
[HypCDE]-Fe(CO)(CN)2 + a [NiFe]-hydrogenase large subunit→{[NiFe]-hydrogenase large subunit}-Fe(CO)(CN)2 + a HypCDE trimer
No genes
{[NiFe]-hydrogenase large subunit}-Fe(CO)(CN)2 + Ni-[HypA hydrogenase accessory protein]→[NiFe]-hydrogenase large subunit with unformed NiFe(CO)(CN)2 cofactor + a HypA hydrogenase accessory protein
b2726 (hypA) +0.1
b2991 (hybF) -0.5
[NiFe]-hydrogenase large subunit with unformed NiFe(CO)(CN)2 cofactor + H2O→holo-[NiFe]-hydrogenase large subunit + an oligopeptide (EC 3.4.23.51)
b2717 (hycI) -0.3
b2993 (hybD) -2.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information