MetaCyc Pathway: superpathway of L-threonine metabolism in Escherichia coli BW25113

Add experiment(s):


Reactions and Genes LB Anaerobic with Nitrofurantoin 0.000015625 mM
remove
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
b3117 (tdcB) +0.1
b3772 (ilvA) +0.3
L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177)
No genes
L-threonine 3-dehydrogenase:
L-threonine + NAD+→L-2-amino-3-oxobutanoate + NADH + H+ (EC 1.1.1.103)
b3616 (tdh) -1.8
L-threonine→acetaldehyde + glycine (EC 4.1.2.48; 4.1.2.5)
b0870 (ltaE) -0.1
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2 (EC 4.1.1.81)
No genes
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10)
b0351 (mhpF) -0.1
b1241 (adhE) -0.6
b2455 (eutE) -0.1
Glycine C-acetyltransferase (in reverse):
L-2-amino-3-oxobutanoate + coenzyme A→glycine + acetyl-CoA (EC 2.3.1.29)
b3617 (kbl) -2.5
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
b3117 (tdcB) +0.1
b3772 (ilvA) +0.3
L-threonine 3-dehydrogenase:
L-2-amino-3-oxobutanoate + H+→aminoacetone + CO2 (spontaneous) (EC 1.1.1.103; 1.1.1.381)
b3616 (tdh) -1.8
Primary-amine oxidase:
aminoacetone + dioxygen + H2O→methylglyoxal + ammonium + hydrogen peroxide (EC 1.4.3.21)
b1386 (tynA) -0.1
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
b3113 (yhaR) -0.2
b3117 (tdcB) +0.1
b3772 (ilvA) +0.3
b4243 (yjgF) -0.5
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
b0078 (ilvH) -0.0
b3670 (ilvN) +0.3
b3671 (ilvB) +0.2
b3769 (ilvM) -0.1
b0077 (ilvI) -0.2
Formate C-acetyltransferase (in reverse):
2-oxobutanoate + coenzyme A→propanoyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW) +0.2
b0903 (pflB) -4.6
b2579 (yfiD) -2.3
b3951 (pflD) +0.0
b3114 (tdcE) -0.4
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
b3774 (ilvC) +0.1
propanoyl-CoA + phosphate→propanoyl phosphate + coenzyme A (EC 2.3.1.222)
b2297 (pta) -0.4
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
b0269 (yagF) +0.2
b4297 (yjhG) -0.0
b3771 (ilvD) +0.1
Acetate kinase (in reverse):
propanoyl phosphate + ADP→propanoate + ATP (EC 2.7.2.1; 2.7.2.14; 2.7.2.15; 2.7.2.7)
b2296 (ackA) N.D.
b3115 (tdcD) -0.0
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
b3770 (ilvE) -0.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information