MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Escherichia coli BW25113
| Reactions and Genes | LB Anaerobic with Nitrofurantoin 0.000015625 mM |
|---|---|
| remove | |
| Glycine oxidase: glycine + dioxygen + H2O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3) | |
| No genes | |
| 3-ethylmalate synthase: glyoxylate + butanoyl-CoA + H2O→3-ethylmalate + coenzyme A + H+ (EC 2.3.3.7) | |
| No genes | |
| 3-isopropylmalate dehydrogenase: 3-ethylmalate + NAD+→2-oxovalerate + CO2 + NADH (EC 1.1.1.85) | |
| b0073 (leuB) | +0.2 |
| 2-oxovalerate→3-methyl-2-oxobutanoate | |
| No genes | |
| Pyruvate decarboxylase: 2-oxovalerate + H+→1-butanal + CO2 (EC 4.1.1.1) | |
| No genes | |
| 1-butanal + NADH + H+→butan-1-ol + NAD+ | |
| No genes | |
| Branched-chain-2-oxoacid decarboxylase: 3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72) | |
| No genes | |
| Alcohol dehydrogenase (in reverse): isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1) | |
| b0325 (yahK) | +0.2 |
| b0356 (frmA) | -0.1 |
| b1241 (adhE) | -0.6 |
| b1478 (adhP) | -0.3 |
| b3253 (yhdH) | -2.3 |
| b4269 (yjgB) | -0.1 |
| b3589 (yiaY) | +0.1 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information