MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Nitrofurantoin 0.000015625 mM
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
b0126 (can) N.D.
b0339 (cynT) -0.1
Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
b3255 (accB) N.D.
b3256 (accC) N.D.
b3973 (birA) +0.3
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
b0185 (accA) N.D.
b2316 (accD) N.D.
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
b1008 (ycdI) -0.2
b1539 (ydfG) +0.2
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
No genes
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
b3253 (yhdH) -2.3
Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H+ + phosphate (EC 6.4.1.3)
b0185 (accA) N.D.
b2316 (accD) N.D.
Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)
b2917 (yliK) +0.2
succinyl-CoA + NADPH + H+→succinate semialdehyde + coenzyme A + NADP+ (EC 1.2.1.76)
No genes
succinate semialdehyde + NADPH + H+→4-hydroxybutanoate + NADP+ (EC 1.1.1.co)
No genes
4-hydroxybutanoate + ATP + coenzyme A→4-hydroxybutanoyl-CoA + AMP + diphosphate (EC 6.2.1.40)
No genes
4-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.120)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
b2341 (yfcX) -0.0
b3846 (fadB) +0.0
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
b2341 (yfcX) -0.0
b3846 (fadB) +0.0
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
b1397 (paaJ) +0.4
b2224 (atoB) +0.1
b2342 (yfcY) +0.1
b2844 (yqeF) -0.2
b3845 (fadA) +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information