MetaCyc Pathway: glyoxylate assimilation in Escherichia coli BW25113

Reactions and Genes	LB Anaerobic with Nitrofurantoin 0.000015625 mM
Carbonate dehydratase (in reverse): CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
b0126 (can)	N.D.
b0339 (cynT)	-0.1
Biotin carboxylase: hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
b3255 (accB)	N.D.
b3256 (accC)	N.D.
b3973 (birA)	+0.3
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
b0185 (accA)	N.D.
b2316 (accD)	N.D.
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
b1008 (ycdI)	-0.2
b1539 (ydfG)	+0.2
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
No genes
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
b3253 (yhdH)	-2.3
Malyl-CoA lyase (in reverse): propanoyl-CoA + glyoxylate→(2R,3S)-β-methylmalyl-CoA (EC 4.1.3.24)
No genes
(2R,3S)-β-methylmalyl-CoA→2-methylfumaryl-CoA + H2O (EC 4.2.1.148)
No genes
2-methylfumaryl-CoA→3-methylfumaryl-CoA (EC 5.4.1.3)
No genes
3-methylfumaryl-CoA + H2O→(3S)-citramalyl-CoA (EC 4.2.1.153)
No genes
Citramalyl-CoA lyase: (3S)-citramalyl-CoA→acetyl-CoA + pyruvate (EC 4.1.3.25)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information