MetaCyc Pathway: anaerobic energy metabolism (invertebrates, cytosol) in Escherichia coli BW25113
| Reactions and Genes | LB Anaerobic with Amoxicillin 0.0000078125 mM |
|---|---|
| remove | |
| Phosphoenolpyruvate carboxykinase (GTP) (in reverse): phosphoenolpyruvate + CO2 + GDP→oxaloacetate + GTP (EC 4.1.1.32) | |
| No genes | |
| Pyruvate kinase (in reverse): phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40) | |
| b1676 (pykF) | +1.7 |
| b1854 (pykA) | +1.3 |
| Phosphoenolpyruvate carboxykinase (GTP) (in reverse): phosphoenolpyruvate + CO2 + IDP→oxaloacetate + ITP (EC 4.1.1.32) | |
| No genes | |
| Alanine transaminase (in reverse): pyruvate + L-glutamate→2-oxoglutarate + L-alanine (EC 2.6.1.2) | |
| b2290 (yfbQ) | -0.5 |
| b2379 (yfdZ) | +0.2 |
| Malate dehydrogenase (in reverse): oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38) | |
| b0801 (ybiC) | +0.8 |
| b1479 (sfcA) | -0.2 |
| b3236 (mdh) | -0.1 |
| Alanine racemase: L-alanine→D-alanine (EC 5.1.1.1; 5.1.1.10) | |
| b1190 (dadX) | -0.4 |
| b1622 (malY) | +0.1 |
| b3008 (metC) | -0.2 |
| b4053 (alr) | -1.5 |
| Aspartate transaminase: 2-oxoglutarate + L-aspartate→L-glutamate + oxaloacetate (EC 2.6.1.1) | |
| b0928 (aspC) | +1.0 |
| b4340 (yjiR) | -0.1 |
Links:
- Pathway details at MetaCyc
- Fitness data for 13 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information