MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Furaltadone 0.000015625 mM
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
b0074 (leuA) -0.3
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
b0071 (leuD) +0.0
b0072 (leuC) +0.1
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
b0071 (leuD) +0.0
b0072 (leuC) +0.1
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
b0073 (leuB) +0.1
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
b0073 (leuB) +0.1
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
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Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) -0.1
b0356 (frmA) +0.2
b1241 (adhE) -1.0
b1478 (adhP) -0.2
b3253 (yhdH) -3.6
b4269 (yjgB) +0.1
b3589 (yiaY) -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information